Coding

Part:BBa_K4606004:Design

Designed by: Baochao Li   Group: iGEM23_Nanjing-NFLS   (2023-09-14)


SMAD4(mutant type)

For detailed design information, you can look up the registry of (BBa_K 4606003), the device for gene segment of SMAD4. According to the base sequence information of SMAD4 in the database, we mutated the SMAD4 gene in a region that complementary binds to miR-3074-5p. By inserting its 3’UTR region (414bp) behind the luciferase gene in the vector, we could determine the expression level of this gene segment.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Based on the online repository of miRNA targets, SMAD4 might be a target of miR-3074-5p in osteoarthritis. Here we mutated the SMAD4 gene (wild type) in a region that complementary binds to miR-3074-5p.

Source

The base sequences were derived from the database of Targetscan website.

References